Gluten intolerance 2in1 (genetic + serological test)

Advantages of the 2in1 test

• Take 2 tests in one - serological + genetic
• Total of 7 parameters measured
• Results within 15 working days
• Identifies the presence of genes associated with gluten intolerance + antibody testing
• Results will allow you to adjust your diet if necessary

What´s measured

Gluten intolerance (gluten)

The advantage of screening for asymptomatic coeliac disease in the general population has not been proven [1-2]. However, expert guidelines suggest that screening for coeliac disease should be considered in asymptomatic first-degree relatives of individuals with a confirmed diagnosis of the condition [1-2]. Serologic testing for coeliac disease is recommended in adults with any of the following symptoms:

• Gastrointestinal symptoms:
  • chronic or recurrent diarrhoea or constipation
  • unexplained weight loss
  • abdominal pain, bloating
• Other symptoms:
  • iron deficiency anemia after excluding other causes
  • fatigue
  • herpetiform dermatitis

Serological examination of gluten

Serological testing, specifically tissue transglutaminase (tTG)-IgA antibody testing, is the preferred method for detecting coeliac disease in adults. In addition, we simultaneously measure total IgA levels. In patients with IgA deficiency, we conduct IgG-based testing using deamidated gliadin peptide (DGP)-IgG. An alternative approach is to conduct both IgA- and IgG-based testing, particularly tTG-IgA and DGP-IgG, especially in patients with a high likelihood of coeliac disease [3-4].

The examination includes

The test package includes the following tests associated with coeliac disease:

• Blood count with differential
  
  • It helps in the assessment of the overall health status, can assist in detecting anemia (low red blood cell count), and thus impaired oxygen supply to tissues, as well as in determining the risk of infections, inflammations, immune disorders, or coagulation problems.
• (tTG)-IgA, (tTG)-IgG
  • Antibodies to tissue transglutaminase, both IgA and IgG classes, are fundamental in the investigation of coeliac disease because the tissue transglutaminase enzyme is directly associated with the disease's pathogenesis.
    
• (DGP)-IgA, (DGP)-IgG
  • Antibodies to deamidated gliadin of both IgA and IgG classes provide a quantitative assessment with greater specificity and sensitivity compared to antibodies to natural gliadin, which were previously used in the diagnosis of coeliac disease.
    
• total IgA.    
  • Immunoglobulins of the IgA class are one of five structurally and functionally distinct classes of antibodies that the body produces in response to encountering foreign or intolerant substances. A distinction is made between serum IgA (present in the blood) and mucosal IgA (present in the mucous membranes of the ducts, especially those of the digestive, respiratory and genitourinary tracts). Examination of the total amount of IgA in the blood makes it possible to correctly interpret the levels of individual antibodies in the IgA class.

• Genetic test for HLA-DQ2 and HLA-DQ8

Genetic test for celiac disease

It has shown that genes from the major histocompatibility complex (MHC) II region are primarily involved in the genetic predisposition to coeliac disease. It's estimated that this complex accounts for up to 40% of the genetic susceptibility to coeliac disease. However, the presence of these genes does not mean that coeliac disease will develop. Around 25–30% of the population carries this genetic makeup, yet only 1% of them are likely to develop the disease. Given that the vast majority of individuals with confirmed coeliac disease (99%) possess one of the DQ2/DQ8 alleles of the HLA system, this test proves valuable for ruling out a predisposition to coeliac disease. A negative test result means that it is virtually impossible for you to develop coeliac disease in your lifetime. [5]